LOCUS       40924_31540       12188 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pMpGWB216 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12188)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T.
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha
  JOURNAL   PLoS ONE 10 (9), E0138876 (2015)
  PUBMED    26406247
REFERENCE   2  (bases 1 to 12188)
  AUTHORS   Nishihama R, Ishizaki K, Kohchi T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2015) Contact:Ryuichi Nishihama Kyoto University, 
            Graduate School of Biostudies; Kitashirakawa-oiwake-cho, Sakyo-ku, 
            Kyoto, Kyoto 606-8502, Japan URL :http://www.lif.kyoto-u.ac.jp/e/
REFERENCE   3  (bases 1 to 12188)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12188)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2015"; volume: "10"; issue: "9"; pages: "E0138876"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-JUN-2015) Contact:Ryuichi Nishihama Kyoto University, Graduate 
            School of Biostudies"; volume: " Kitashirakawa-oiwake-cho, Sakyo-ku,
            Kyoto, Kyoto 606-8502, Japan URL :http"; pages: 
            "//www.lif.kyoto-u.ac.jp/e"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12188
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    323..447
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        484..514
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             568..1224
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1569..1871
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1915..2039)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2050..3477
                     /codon_start=1
                     /label=tdTomato
                     /note="tandem dimeric (pseudo-monomeric) derivative of
                     DsRed (Shaner et al., 2004)"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             3484..3504
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      3529..3781
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3815..3831)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3839..3855
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3863..3893)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3908..3929)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     polyA_signal    complement(4014..4186)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4246..4776)
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MVRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(4839..5515)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    complement(5710..5734)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6255..7043
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7292..7880
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8066..8206)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8550..8744)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8813..9883)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10315..10941)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagagttat caacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      361 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      421 taaaacacaa catatccagt cactatggcg gcccctcgag gggccgcatt aggcacccca
      481 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccggcg
      541 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
      601 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
      661 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
      721 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
      781 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
      841 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
      901 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
      961 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     1021 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     1081 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     1141 catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     1201 tgcgatgagt ggcagggcgg ggcgtaaacg cgtggatccg gcttactaaa agccagataa
     1261 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
     1321 cgaagtatgt caaaaagagg tgtgctatga agcagcgtat tacagtgaca gttgacagcg
     1381 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     1441 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     1501 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacaggga
     1561 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     1621 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
     1681 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
     1741 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     1801 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     1861 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
     1921 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     1981 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt
     2041 gataacagca tggtgagcaa gggcgaggag gtcatcaaag agttcatgcg cttcaaggtg
     2101 cgcatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     2161 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc
     2221 gcctgggaca tcctgtcccc ccagttcatg tacggctcca aggcgtacgt gaagcacccc
     2281 gccgacatcc ccgattacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     2341 atgaacttcg aggacggcgg tctggtgacc gtgacccagg actcctccct gcaggacggc
     2401 acgctgatct acaaggtgaa gatgcgcggc accaacttcc cccccgacgg ccccgtaatg
     2461 cagaagaaga ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg
     2521 ctgaagggcg agatccacca ggccctgaag ctgaaggacg gcggccacta cctggtggag
     2581 ttcaagacca tctacatggc caagaagccc gtgcaactgc ccggctacta ctacgtggac
     2641 accaagctgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgagcgc
     2701 tccgagggcc gccaccacct gttcctgggg catggcaccg gcagcaccgg cagcggcagc
     2761 tccggcaccg cctcctccga ggacaacaac atggccgtca tcaaagagtt catgcgcttc
     2821 aaggtgcgca tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag
     2881 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg
     2941 cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccaaggc gtacgtgaag
     3001 caccccgccg acatccccga ttacaagaag ctgtccttcc ccgagggctt caagtgggag
     3061 cgcgtgatga acttcgagga cggcggtctg gtgaccgtga cccaggactc ctccctgcag
     3121 gacggcacgc tgatctacaa ggtgaagatg cgcggcacca acttcccccc cgacggcccc
     3181 gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac
     3241 ggcgtgctga agggcgagat ccaccaggcc ctgaagctga aggacggcgg ccgctacctg
     3301 gtggagttca agaccatcta catggccaag aagcccgtgc aactgcccgg ctactactac
     3361 gtggacacca agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     3421 gagcgctccg agggccgcca ccacctgttc ctgtacggca tggacgagct gtacaagttg
     3481 cagcctaaga agaagagaaa ggttggagga tagagctcga atttccccga tcgttcaaac
     3541 atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata
     3601 taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt
     3661 atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac
     3721 aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat
     3781 cgggaattgg ttccggaacc aattcgtaat catggtcata gctgtttcct gtgtgaaatt
     3841 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     3901 gtgcctaatg agtgagctaa ctcacattag gctgaattag gcgcgcctat ttctgaattc
     3961 aacacattgc ggacgttttt aatgtactga attaacgccg aattaattcg ggggatctgg
     4021 attttagtac tggattttgg ttttaggaat tagattttat tgatagaagt attttacaaa
     4081 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     4141 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga
     4201 tcgactctag gccgggaagc cgatctcggc ttgaacgaat tgttaggtgg cggtacttgg
     4261 gtcgatatca aagtgcatca cttcttcccg tatgcccaac tttgtataga gagccactgc
     4321 gggatcgtca ccgtaatctg cttgcacgta gatcacataa gcaccaagcg cgttggcctc
     4381 atgcttgagg agattgatga gcgcggtggc aatgccctgc ctccggtgct cgccggagac
     4441 tgcgagatca tagatataga tctcactacg cggctgctca aacttgggca gaacgtaagc
     4501 cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca agcgcgatga atgtcttact
     4561 acggagcaag ttcccgaggt aatcggagtc cggctgatgt tgggagtagg tggctacgtc
     4621 tccgaactca cgaccgaaaa gatcaagagc agcccgcatg gatttgactt ggtcagggcc
     4681 gagcctacat gtgcgaatga tgcccatact tgagccacct aactttgttt tagggcgact
     4741 gccctgctgc gtaacatcgt tgctgctgcg taccatggtc gatcgacaga tctgcgaaag
     4801 ctcgagagag atagatttgt agagagagac tggtgatttc agcgtgtcct ctccaaatga
     4861 aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc
     4921 cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt tggaacgtct
     4981 tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag
     5041 gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc
     5101 ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt
     5161 cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct
     5221 ttgatattct tggagtagac gagagtgtcg tgctccacca tgttcacatc aatccacttg
     5281 ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc
     5341 atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg
     5401 atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc
     5461 tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc
     5521 cctttggtct tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc
     5581 caccatgttg gcaagctgct ctagccaata cgcaaaccga attcagaaat aaattcagcc
     5641 taattcggcg ttaattcagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag
     5701 cgtcaatttg tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg
     5761 gcagctcggc acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag
     5821 cgggagagcc gttgtaaggc ggcagacttt gctcatgtta ccgatgctat tcggaagaac
     5881 ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg
     5941 taacgatgac agagcgttgc tgcctgtgat caattcgggc acgaacccag tggacataag
     6001 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     6061 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     6121 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     6181 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     6241 caaagttaaa catcatgggg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     6301 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     6361 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     6421 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     6481 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     6541 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     6601 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     6661 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     6721 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     6781 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     6841 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     6901 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     6961 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     7021 agatcaccaa ggtagtcggc aaataatgtc tagctagaaa ttcgttcaag ccgacgccgc
     7081 ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa
     7141 actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat
     7201 tgggagatat atcatgcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     7261 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7321 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7381 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     7441 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     7501 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     7561 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     7621 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     7681 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     7741 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     7801 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     7861 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     7921 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     7981 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     8041 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     8101 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     8161 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
     8221 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     8281 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     8341 gaggcagggt gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc
     8401 gccctggtag attgcctggc cgtaggccag ccatttttga gcggccagcg gccgcgatag
     8461 gccgacgcga agcggcgggg cgtagggagc gcagcgaccg aagggtaggc gctttttgca
     8521 gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt ttaagagttt
     8581 taataagttt taaagagttt taggcggaaa aatcgccttt tttctctttt atatcagtca
     8641 cttacatgtg tgaccggttc ccaatgtacg gctttgggtt cccaatgtac gggttccggt
     8701 tcccaatgta cggctttggg ttcccaatgt acgtgctatc cacaggaaag agaccttttc
     8761 gacctttttc ccctgctagg gcaatttgcc ctagcatctg ctccgtacat taggaaccgg
     8821 cggatgcttc gccctcgatc aggttgcggt agcgcatgac taggatcggg ccagcctgcc
     8881 ccgcctcctc cttcaaatcg tactccggca ggtcatttga cccgatcagc ttgcgcacgg
     8941 tgaaacagaa cttcttgaac tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca
     9001 accatctggc ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga aaacggccga
     9061 tgccgggatc gatcaaaaag taatcggggt gaaccgtcag cacgtccggg ttcttgcctt
     9121 ctgtgatctc gcggtacatc caatcagcta gctcgatctc gatgtactcc ggccgcccgg
     9181 tttcgctctt tacgatcttg tagcggctaa tcaaggcttc accctcggat accgtcacca
     9241 ggcggccgtt cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa
     9301 tgcaggtttc taccaggtcg tctttctgct ttccgccatc ggctcgccgg cagaacttga
     9361 gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt gtctcccttc ccttcccggt
     9421 atcggttcat ggattcggtt agatgggaaa ccgccatcag taccaggtcg taatcccaca
     9481 cactggccat gccggccggc cctgcggaaa cctctacgtg cccgtctgga agctcgtagc
     9541 ggatcacctc gccagctcgt cggtcacgct tcgacagacg gaaaacggcc acgtccatga
     9601 tgctgcgact atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa tctggttgct
     9661 cgtcgccctt gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt
     9721 ctttgcggat tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct tctgcctcga
     9781 tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc caccaggtca tcacccagcg
     9841 ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgct cacgccgatt cctcgggctt
     9901 gggggttcca gtgccattgc agggccggca gacaacccag ccgcttacgc ctggccaacc
     9961 gcccgttcct ccacacatgg ggcattccac ggcgtcggtg cctggttgtt cttgattttc
    10021 catgccgcct cctttagccg ctaaaattca tctactcatt tattcatttg ctcatttact
    10081 ctggtagctg cgcgatgtat tcagatagca gctcggtaat ggtcttgcct tggcgtaccg
    10141 cgtacatctt cagcttggtg tgatcctccg ccggcaactg aaagttgacc cgcttcatgg
    10201 ctggcgtgtc tgccaggctg gccaacgttg cagccttgct gctgcgtgcg ctcggacggc
    10261 cggcacttag cgtgtttgtg cttttgctca ttttctcttt acctcattaa ctcaaatgag
    10321 ttttgattta atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc
    10381 tgattcaaga acggttgtgc cggcggcggc agtgcctggg tagctcacgc gctgcgtgat
    10441 acgggactca agaatgggca gctcgtaccc ggccagcgcc tcggcaacct caccgccgat
    10501 gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct tgtagccttc catccgtgac
    10561 ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg gcccatatgt cgtaagggct
    10621 tggctgcacc ggaatcagca cgaagtcggc tgccttgatc gcggacacag ccaagtccgc
    10681 cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg
    10741 gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt tgatcttccc gcacggccgc
    10801 ccaatcgcgg gcactgccct ggggatcgga atcgactaac agaacatcgg ccccggcgag
    10861 ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt
    10921 aagtacagcg ataaccttca tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc
    10981 atatacgcag cgaccgcatg acgcaagctg ttttactcaa atacacatca cctttttaga
    11041 cggcggcgct cggtttcttc agcggccaag ctggccggcc aggccgccag cttggcatca
    11101 gacaaaccgg ccaggatttc atgcagccgc acggttgaga cgtgcgcggg cggctcgaac
    11161 acgtacccgg ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc
    11221 tggccgtcct ggtgcggttt catgcttgtt cctcttggcg ttcattctcg gcggccgcca
    11281 gggcgtcggc ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg
    11341 gcgtcacttc ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca
    11401 gtgcagccgc ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga
    11461 tctgtgccgg ggtgagggta gggcgggggc caaacttcac gcctcgggcc ttggcggcct
    11521 cgcgcccgct ccgggtgcgg tcgatgatta gggaacgctc gaactcggca atgccggcga
    11581 acacggtcaa caccatgcgg ccggccggcg tggtggtgtc ggcccacggc tctgccaggc
    11641 tacgcaggcc cgcgccggcc tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc
    11701 tgcgggccag gcggtctagc ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa
    11761 gcatcctggc cagctccggg cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct
    11821 tggtgcagcc ggccgcgtgc agttcggccc gttggttggt caagtcctgg tcgtcggtgc
    11881 tgacgcgggc atagcccagc aggccagcgg cggcgctctt gttcatggcg taatgtctcc
    11941 ggttctagtc gcaagtattc tactttatgc gactaaaaca cgcgacaaga aaacgccagg
    12001 aaaagggcag ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag
    12061 aagacggctg cactgaacgt cagaagccga ctgcactata gcagcggagg ggttggatca
    12121 aagtactttg atcccgaggg gaaccctgtg gttggcatgc acatacaaat ggacgaacgg
    12181 ataaacct
//
